The Anatomy of a Leading Case: \u3cem\u3eLawrence v. Fox\u3c/em\u3e in the Courts, the Casebooks, and the Commentaries

In spite of the wide diversity of training, practice, and location of lawyers throughout the United States, virtually all share one experience: the standard core curriculum of the first year of law school taught by the case method. The extent to which that experience in parsing cases in contracts, torts, and property shapes the American legal mentality is open to debate, but it undeniably has an impact. The first-year experience socializes law students in the culture of the law. During this period, students learn the language of the law and the ways that lawyers think. During this period, too, students absorb certain basic notions about legal analysis and the shape of the legal system, and begin to view the world as common lawyers. Included among these basic notions, the conventional wisdom of basic law training, is the concept of the leading case. This Article explores the notion of the leading case, and places the concept in an historical and jurisprudential framework

Gap junction-mediated glycinergic inhibition ensures precise temporal patterning in vocal behavior

Precise neuronal firing is especially important for behaviors highly dependent on the correct sequencing and timing of muscle activity patterns, such as acoustic signaling. Acoustic signaling is an important communication modality for vertebrates, including many teleost fishes. Toadfishes are well known to exhibit high temporal fidelity in synchronous motoneuron firing within a hindbrain network directly determining the temporal structure of natural calls. Here, we investigated how these motoneurons maintain synchronous activation. We show that pronounced temporal precision in population-level motoneuronal firing depends on gap junction-mediated, glycinergic inhibition that generates a period of reduced probability of motoneuron activation. Super-resolution microscopy confirms glycinergic release sites formed by a subset of adjacent premotoneurons contacting motoneuron somata and dendrites. In aggregate, the evidence supports the hypothesis that gap junction-mediated, glycinergic inhibition provides a timing mechanism for achieving synchrony and temporal precision in the millisecond range for rapid modulation of acoustic waveforms

ЭКСПРЕССИЯ CXCR4 В РАЗЛИЧНЫХ ПОПУЛЯЦИЯХ ЦИРКУЛИРУЮЩИХ И ОДИНОЧНЫХ ОПУХОЛЕВЫХ КЛЕТОК РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ

The aim of this study was to assess CXCR4 expression in different subsets of CTCs and single (detached) breast cancer cells.Materials and methods. Thirty five patients with invasive breast carcinoma of no specialtype (IC NST) (T1-4N0-2M0), between 29 and 69 years of age were included in this study. Different subsets of CTCs with CXCR4 expression were evaluated by flow cytometry. A  confocal microscopy was used to assess CXCR4 expression in different subsets of single (detached) cancer cells in breast tissue.Results. The CXCR4 was expressed in CTCs without stem-like and EMT phenotype, in CTCs  with EMT but not stem markers and in stem-like CTCs without EMT features. In all blood  samples, the CXCR4 expression in CTCs with stem-like and EMT phenotype was absent. In  breast tumor the CXCR4 was expressed in the non stemlike single (detached) breast cancer  cells with EMT features, in the single (detached) breast cancer cells with stem and EMT  features. In all tumor samples the stem-like or non stem-like single (detached) breast  cancer cells without EMT features were absent.Conclusions. Different subsets of the CTCs exhibited CXCR4. The CXCR4 expression did not  depend on the presence or absence of stem or/and EMT features in tumor cells. We showed that some subsets of single (detached) breast cancer cells in the primary tumor  were characterized by the ability to express CXCR4 and may be a source of the respective CTC subsets.Целью исследования явилось определение экспрессии CXCR4 в различных популяциях циркулирующих (ЦОК) и одиночных (дискретных) опухолевых клеток рака молочной железы.Материал и методы. В исследование были включены 35 пациенток с инвазивной карциномой неспецифического типа молочной железы (T1–4N0–2M0) в возрасте от 29 до 69 лет. Экспрессию CXCR4 в  различных популяциях ЦОК оценивали методом проточной цитометрии. Для оценки экспрессии CXCR4 в  аналогичных популяциях одиночных (дискретных) опухолевых клеток в первичной опухоли использовали метод конфокальной микроскопии.Результаты. Нами было установлено, что CXCR4 экспрессировался ЦОК без признаков стволовости и  эпителиально-мезенхимального перехода (ЭМП), ЦОК с признаками ЭМП, но без маркеров стволовости, а  также ЦОК с признаками стволовости, но без признаков ЭМП. У всех пациенток в крови ЦОК с признаками  стволовости и ЭМП не экспрессировался CXCR4. В первичной опухоли молочной железы CXCR4  обнаруживался как на одиночных (дискретных) опухолевых клетках без признаков стволовости с  признаками ЭМП, так и на клетках с маркерами стволовости и ЭМП. У всех пациенток в образцах первичной  опухоли отсутствовали стволовые и нестволовые клетки без признаков ЭМП.Заключение. Таким образом, CXCR4 экспрессируются на различных популяциях ЦОК. Экспрессия CXCR4  не зависит от наличия или отсутствия признаков стволовости и/или ЭМП в опухолевых клетках. Также мы  показали, что некоторые популяции одиночных (дискретных) опухолевых клеток в первичной опухоли  характеризуются способностью презентировать на своей мембране CXCR4 и могут являться источником соответствующих популяций ЦОК

On the nature of the brightest globular cluster in M81

We analyse the photometric, chemical, star formation history and structural properties of the brightest globular cluster (GC) in M81, referred to as GC1 in this work, with the intention of establishing its nature and origin. We find that it is a metal-rich ([Fe/H] = -0.60 +/- 0.10), alpha-enhanced ([alpha/Fe] similar to 0.20 +/- 0.05), core-collapsed (core radius r(c) = 1.2 pc, tidal radius r(t) = 76r(c)), old (> 13 Gyr) cluster. It has an ultraviolet excess equivalent of similar to 2500 blue horizontal branch stars. It is detected in X-rays indicative of the presence of low-mass binaries. With a mass of 1.0 x 10(7) M-circle dot, the cluster is comparable in mass to M31-G1 and is four times more massive than omega Cen. The values of r(c), absolute magnitude and mean surface brightness of GC1 suggest that it could be, like massive GCs in other giant galaxies, the left-over nucleus of a dissolved dwarf galaxy

The Anatomy of a Leading Case: \u3cem\u3eLawrence v. Fox\u3c/em\u3e in the Courts, the Casebooks, and the Commentaries

In spite of the wide diversity of training, practice, and location of lawyers throughout the United States, virtually all share one experience: the standard core curriculum of the first year of law school taught by the case method. The extent to which that experience in parsing cases in contracts, torts, and property shapes the American legal mentality is open to debate, but it undeniably has an impact. The first-year experience socializes law students in the culture of the law. During this period, students learn the language of the law and the ways that lawyers think. During this period, too, students absorb certain basic notions about legal analysis and the shape of the legal system, and begin to view the world as common lawyers. Included among these basic notions, the conventional wisdom of basic law training, is the concept of the leading case. This Article explores the notion of the leading case, and places the concept in an historical and jurisprudential framework